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amplification efficiency  (DSMZ)


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    DSMZ amplification efficiency
    Amplification Efficiency, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amplification+efficiency/Methanosarcina+barkeri/bio_rxiv__64898__2026__03__29__715081-115-20-18
    Average 95 stars, based on 123 article reviews
    amplification efficiency - by Bioz Stars, 2026-09
    95/100 stars

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    Generated:

    Article Title: Invasive plant soil legacies shape microbial function and community organization under short-term carbon and nitrogen amendments
    Article Snippet: .. Standard curves were generated from tenfold serial dilutions (10−1 to 10−6) of Methanosarcina barkeri genomic DNA (DSM 800, DSMZ), with amplification efficiency of 106.5% and R2 = 0.99. ..

    Amplification:

    Article Title: Invasive plant soil legacies shape microbial function and community organization under short-term carbon and nitrogen amendments
    Article Snippet: .. Standard curves were generated from tenfold serial dilutions (10−1 to 10−6) of Methanosarcina barkeri genomic DNA (DSM 800, DSMZ), with amplification efficiency of 106.5% and R2 = 0.99. ..



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    Fig. 5. Sebastes ruberrimus target assay design visualization. A) Unikseq output butterfly plot of the relevant regions for the design of the <t>eSERU5</t> targeted qPCR assay. B) eSERU5 amplicon alignment. Note that the eSERU5 region is out of range to align with most other species as well as human, cat, and dog sequences. In situ validation confirmed that species of concern were not amplified by this assay. There is no intraspecific variability in any of the key assay regions. See the Fig. 3 legend for more details.
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    Fig. 5. Sebastes ruberrimus target assay design visualization. A) Unikseq output butterfly plot of the relevant regions for the design of the eSERU5 targeted qPCR assay. B) eSERU5 amplicon alignment. Note that the eSERU5 region is out of range to align with most other species as well as human, cat, and dog sequences. In situ validation confirmed that species of concern were not amplified by this assay. There is no intraspecific variability in any of the key assay regions. See the Fig. 3 legend for more details.

    Journal: Ecological Indicators

    Article Title: Comparison of environmental DNA and SCUBA diving methods to survey keystone rockfish species on the Central Coast of British Columbia, Canada

    doi: 10.1016/j.ecolind.2024.111830

    Figure Lengend Snippet: Fig. 5. Sebastes ruberrimus target assay design visualization. A) Unikseq output butterfly plot of the relevant regions for the design of the eSERU5 targeted qPCR assay. B) eSERU5 amplicon alignment. Note that the eSERU5 region is out of range to align with most other species as well as human, cat, and dog sequences. In situ validation confirmed that species of concern were not amplified by this assay. There is no intraspecific variability in any of the key assay regions. See the Fig. 3 legend for more details.

    Article Snippet: However, the eSERU5 amplification efficiency at 78 % (Table 1) is slightly lower than the 80 % recommended by the new Canadian national standard on performance criteria for the analysis of eDNA by targeted qPCR (Abbott et al., 2023).

    Techniques: Amplification, In Situ, Biomarker Discovery